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In Open Society " Privolzhsknefteprovod " the method biorecultivation the petropolluted grounds for the first time is applied.

New for Privolzhsk workers the way of recultivation allows to minimize damage to an environment from possible outflow of oil. In a basis of biorecultivation the polluted oil of the ground application complex processings of ground by a preparation "Devouroil", mineral fertilizers and the subsequent crop on the processed areas of long-term grasses lays. In 2007, according to the Program of ecological safety of Open Society "Privolzhsknefteprovod", biorecultivation for the first time it has been applied on the grounds polluted by oil by the area of 4,4 hectares. In May the ground has been processed by a solution "Devouroil", then watered by ammoniac saltpeter and superphosphate. In June-July the ground was as is processed again. In August on the processed ground sowed Lucerne, porridge.

The tests were conducted by the Hong Kong Polytechnic University, in the Water and Waste Laboratory. Here are the certificates in English and Chinese for your reference. DEVOROIL treatment hit an impressive 71% oil degradation in only 4 weeks!!! The original rate of pollution was intentionally set at the highest level ever known up till now: 50% crude oil in the soil. This test simulated - and actually emphasized - the extreme situations occurring after major oil spills. After the latest European major spills, Erika or Prestige, the use of classical chemicals took up to 7 years to get the result DEVOROIL obtained in 4 weeks only. DEVOROIL has proved again to be the most efficient natural agent for hydrocarbons bioremediation on the market.

Laboratory in charge Water and Waste Laboratory Department of Civil and Structural Engineering
The Hong Kong Polytechnic University Fax: (852) 2334-6389Tel: (852) 2766-6075
E-mail: cewchu@polyu.edu.hk
18 January 2008

Certificate of registration,
Registered in State anagraph GSTR RK at December 30, 2005. № KZ 7100000.06.09.00805

Customer: TOO “Trans-Biznes”
Ungaliev Malik Temirtasovich +7 (7122) 20-38-94; +7 701-511-17-89;
Name of sample: soil (Beinau city)
Date of work: Beginning-10.04.07. Finishing - 10.05.07.

№	Name	Unit measure	Result	Standarts of limits, method for determination
1.	Oil products (10.04.07)	mg/kg	78 425	IR - spectrometry
2.	Oil products (20.04.07)	mg/kg	28 225	IR - spectrometry
3.	Oil products (30.04.07)	mg/kg	6 012	IR – spectrometry
4.	Oil products (10.05.07)	mg/kg	98	IR - spectrometry

VIESOSION ISTAIGOS “GRUNTO VALYMO TECHNOLOGIJOS” KLAIPEDOS FILIALAS
21.02.2007 tel/fax: (5) 268301 (46) 444375

Public office “Technologii po ochistke gruntov” makes biological cleaning of oil polluted soil.
We do it since 1995. We clean 10000 tones of soil polluted by diesel oil, crude oil, masut, machine oil and other products of petroleum refining.

Biotechnology of cleaning with optimal temperature provides using of preparation, feeding components, periodic cultivation, keeping of optimal dampness. On the basis of long time experience of cleaning by Devoroil, we can state that Devoroil is high-performance destructor of broad-spectrum oil pollutions. Devoroil does not have deleterious effect.

In our opinion, LLC “Microbic technologies” creating Devoroil made a grate contribution in cleaning of oil pollutions and development of ecology.

We've been cleaning water from a waste oil products sump. The basic suspension of Devoroil preparation was prepared according to the instruction. Ready suspension was sprayed on the sewage surface. Before cleaning, the water is of black color, it has weighed substances in its structure, the surface is covered by an oil film. The content of oil products is 21,45 mg/l. After cleaning water got transparent, the black and light brown flocculent sediment formed at the bottom. The content of oil products is 0,97 mg/l.

After single processing about 60 square meters of the 20 mm black oil film on water surface by the water suspension of the Devoroil biological preparation in three weeks the black oil film has completely disappeared, and the water table became almost totally clean. The environment temperature thus varied within 15 - 27°C. We think that alongside with mechanical means of cleaning the water surface from black oil, it's effectively to use microbiological method too.

Environment preservation scientific - industrial center (Yaroslavl city)

We inform you of the results on cleaning petropolluted soil by the Devoroil biological product on the territory of Ivanovo station Locomotive depot.

About 1000 cubic meters of the petropolluted soil was a subject to cleaning. One of the three planned processing stages was conducted. The analyses results testify that even a single application of the Devoroil preparation provided reduction of the aliphatic, aromatic hydrocarbons and light tars respectively by 33,26 and 63 %.

The total contents of oil products in a 0 - 30 sm layer decreased by 10 %, and in the top 0 - 10 sm layer by 40 %. The next processing we'll conduct in summer.

During the spring - summer 2005 in the petropolluted territories of 10 locomotive and 6 wagon depots of the South-Ural railway - owned by the Open Society "RJD"subsidiary, cleaning works on petropolluted soils were conducted using the biological product Devoroil.

The subject to processing was 2 thousand tons of polluted soil from the 5 thousand sq. m. area. The initial content of the oil products was 20 - 40 g/kg. The number of cleanings with use of mineral fertilizers was 1 - 2 depending on local conditions. The results of soils cleaning at the enterprises where cleaning is finished averaged between 60 - 80 %. The highest effect was achieved at second processing.

The South-Ural railway is going to continue with the use of the specified preparation.

Brest branch of the Belarusian Railway:

As a result of reclamation Biopreparations "Devoroyl" when the oil content of the soil at a depth of up to 60 cm reduction of petroleum products reached 96.5%. The soil to be used in construction, with podsypke roads and flower for the breakdown.

Cleaning of sumps at mine, sludge ponds and residue settling pits cleaning constructions at the black oil pump station #1 (gas workshop) was conducted. In the same workshop the Devoroil preparation approbation was made on a oil products sludge pond. The first positive results are achieved, this work will be continued. A greater work is done on the sludge pond site and the drain channel of magnesia products DPO workshop #1.

EXTRACT FROM THE LABORATORY RESEARCHES REPORT ON PREPARATIONS ON THE BASIS OF PETROOXIDIZING MICROORGANISMS

Tests were made in April, May 2002 in a laboratory of oil microbiology of the microbiology Institute at the Russian Academy of Science. The soil for the tests was taken from an oil overflow place in Saratov city. The pollution degree is 157 g/kg. Age of pollution is 8 months.

Before the tests the soil was carefully mixed, all foreign particles were removed. Soil ditches (600) have been processed twice (in two weeks) by the preparations and isolates of petrooxidizing microorganisms under the following scheme:

Before processing by the biological products fertilizers (nitrophoska) were added, the soil was regularly humidified and stirred. The contents of the oil products were determined on concentration meter KN-2 (by the method of infra-red spectroscopy). The test results are in the below table.

The biological products and isolates of native oil cultures-destructors efficiency, 2002.

Variant	The initial content of oil, g/kg	The content of oil in 30 days, g/kg	Destructive activity, %
The control (unprocessed by the biological products soil)	157	145	7,6
The Destroil preparation	157	99	36,9
The Devoroil preparation	157	37	76,3
The Roder preparation	157	118	24,8
The isolate 1-8 (exc. in 2001 Chelyabinsk city)	157	102	35,0
The Olevorin preparation	157	103	34,4
The isolate 2-18 (exc. in 2002 Saratov city on an overflow place)	157	85	45,9

Within 30 days under the biological preparations influence from 24,8 to 76,3 % of oil products decayed. From the isolates a little bit more active was isolate 2-18, excreted in 2002 in Saratov city on an overflow place; under its influence 45,9 % of hydrocarbons decayed.

We are sending you an extract from the laboratory researches report on the petrooxidizing microorganisms preparations.

DEVOROIL has shown one of the best results. The preparation action was so effective, that the result could be observed even visually. We hope for the further cooperation.

On August, 19th, 2005 in Oktabrskoe village in the result of not authorized cutting in the Orenburg - Salavat - Ufa (77 km) condensate line there was an emergency emission of gas condensate. About 145 m3 of gas condensate has been thrown out to the atmosphere. The major part of the pollution got into Gumbet river. The adjoining territory - inundated meadows undergone the pollution, the pollution has extended to more than 5 km.

Due to the operative actions of the Orenburg militarized unit of the "Orenburggazprom" Open Company and the experts from UESP "Orenburggazprom" Open Company the condensate outflow has been eliminated and were quickly undertaken the prime actions on localization and collecting of the condensate.

The experts of the "Orenburggazprom" Open Company, UESPand the scientific and technological centre "Prombezopasnost-Orenburg" accepted a decision to use the biological method of additional cleaning of the polluted remote territory.

For the best results special actions have been taken: the natural microflora was preliminary activated by entering humin substances and massive stage-by-stage processing of the polluted territory by the activated biological product Devoroil.

The biological product was preliminary activated by continuous aeration in the water environment at presence of biogenic additives for restoration of the microorganisms’ activity.

4 processing stages were conducted each with 6-10 days break. For acceleration of the condensate pollution oxidation process the doze of the preparation and biogenic additives has been increased in 2,5 times.

Results of visual inspection of the territory showed that in 4 days there appeared the attributes of active destruction of the condensate film. In 12-16 days the condensate film was almost completely destroyed. Active growth of seaweed was marked at the former polluted territory.

The chemical-analytical control has shown that reduction efficiency of oil products dissolved in water has exceeded 90 %.

For protection of water pool on Nerungrinskaya hydroelectric power station are annually carried out more than 10 various actions. One of the most significant application of biological product Devouroil was for clearing a water basin from masut dissolved in thickness of water. The preparation is culture of alive microorganisms which basis of feed is masut.

The preparation has been entered into hydrosystem of Nerungrinskaya hydroelectric power station in 2002, as a result in 2003 was not observed excess of maximum concentration limit on black oil in hydrosystem, water objects of area did not become soiled. Application of a preparation is coordinated with sanitary epidemiological service as it does not render harmful influence on alive organisms.

Biological recycling of petrocontaining waste in the «Moscow NPZ» Open Society

The recycling technology of oil slimes is carried out at the factory as follows. The oil slime is taken to a specially equipped slime detoxification site of 0,8 hectares. The site is diked and in the has a waterproof layer in the basement to prevent the filtrate migration which contains dissolved oil products, to water bearing horizons of spreading soils. Each site map receives structurator-ameliorant sawdust and evenly distributed on the surface with the layer of 15-20 sm. Then the delivered oil slime is mixed up with the stucturator. The general height of the prepared layer does not exceed 0,4 m.

During the process of maps formation the oil slime is processed with the biological product Devoroil and the received substratum is carefully stired. For microorganisms' better oxidizing ability mineral fertilizers (diammophos) are also brought into the maps.

The slime is periodically humidified (2 times a week, or more often) and 2 times a week it's stirred for better aeration conditions for the microorganisms.

For the time of the oil slimes recycling technology use the factory carried out detoxification of about 3500 m3 of oil slime. The new product of the factory is applied basically for building lawns on its territory, and also for leveling roughness, for beautiful landscapes.

Preparation	Devouroil	Valentis	Destroil	Dizoil	Oleovorin
Manufacturer	LLC Microbic Technologies	Closed Company Biotech-Japan	Berdsk preparation factory	LLC Salut-Technologies-West	LLC Bigor
Structure of a preparation	Polyculture	Monoculture	Monoculture	Monoculture	Monoculture
Use form	Powder	Powder	Powder	Powder	Suspension
Titre (kg/gr)	10 exp 8	10 exp 8	10 exp 8	10 exp 7	10 exp 8
Ability to work in thickness of mineral oil	+++	-	-	-	-
Ability to oxidize heavy mineral oil	+++	+	+	+	+
Ability to work at pollution >10%	+++	+	-	+	-
Galotolerancy (% NaCI)	3 - 8 %	-	-	< 3 %	-
Temperature interval of work С	5 - 45	5 - 40	5 - 40	5 - 40	5 - 40
Quantity of a preparation (kg) for degradation of 1t of oil	1	100 - 200	20 - 45	129	166 liters

INMI RAN Professor I. Borzenkov
Ltd. "Microbial Technology" Professor M. Pospelov